RNF20 antibody (N-Term)

Catalog No. ABIN6991864

Product Details anti-RNF20 Antibody

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Binding Specificity: AA 130-180, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Multiple isoforms of RNF20 are known to exist.

Purification: RNF20 Antibody is affinity chromatography purified via peptide column.

Immunogen: RNF20 antibody was raised against a 19 amino acid peptide near the amino terminus of human RNF20. The immunogen is located within amino acids 130 - 180 of RNF20.

Isotype: IgG

Application Details

Application Notes: RNF20 antibody can be used for detection of RNF20 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RNF20 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RNF20 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for RNF20

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Alternative Name: RNF20 (RNF20 Products)

Synonyms: 4833430L21Rik antibody, AW540162 antibody, C79397 antibody, mKIAA4116 antibody, BRE1 antibody, BRE1A antibody, hBRE1 antibody, ring finger protein 20 antibody, RNF20 antibody, Rnf20 antibody

Background: RNF20 Antibody: RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.

Molecular Weight: Predicted: 99, 107 kDa

Observed: 100, 110 kDa

Gene ID: 56254

NCBI Accession: NP_062538

UniProt: Q5VTR2