GOLGA5 antibody (C-Term)

Catalog No. ABIN6991873

Product Details anti-GOLGA5 Antibody

Target: GOLGA5 (Golgin A5 (GOLGA5))

Binding Specificity: AA 540-590, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOLGA5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: GOLGA5 antibody is human, mouse and rat reactive. Two isoforms of GOLGA5 exist due to alternative splicing events.

Purification: GOLGA5 Antibody is affinity chromatography purified via peptide column.

Immunogen: GOLGA5 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human GOLGA5. The immunogen is located within amino acids 540 - 590 of GOLGA5.

Isotype: IgG

Application Details

Application Notes: GOLGA5 antibody can be used for detection of GOLGA5 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: GOLGA5 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: GOLGA5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for GOLGA5

Target: GOLGA5 (Golgin A5 (GOLGA5))

Alternative Name: GOLGA5 (GOLGA5 Products)

Synonyms: GOLIM5 antibody, RFG5 antibody, ret-II antibody, golim5 antibody, ret-ii antibody, rfg5 antibody, Ret-II antibody, im:7153094 antibody, sb:cb898 antibody, wu:fd50h11 antibody, zgc:66400 antibody, golgin A5 antibody, golgin A5 L homeolog antibody, golgi autoantigen, golgin subfamily a, 5 antibody, GOLGA5 antibody, golga5.L antibody, golga5 antibody, Golga5 antibody

Background: GOLGA5 Antibody: GOLGA5 (Golgi autoantigen, subfamily A, member 5), also known as GOLGA84, RFG5 (RET-fused gene 5 protein) or GOLIM5, is a ubiquitously expressed single-pass type II coiled-coil membrane protein that is involved in maintaining Golgi structure (1). It is localized to the Golgi apparatus and predominantly found on membranes at the cis side of the Golgi stack (1,2). As a mitotic phosphoprotein, GOLGA5 binds to active Rab 1 and associates with CASP (an isoform of CDP) in a golgin-tethering complex that is believed to play a role in intra-Golgi retrograde transport (3,4). Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) (5).

Molecular Weight: Predicted: 64, 80 kDa

Observed: 65, 78 kDa

Gene ID: 9950

NCBI Accession: NP_005104

UniProt: Q8TBA6