DAP antibody (C-Term)

Catalog No. ABIN6991874

The Rabbit Polyclonal anti-DAP antibody is suitable to detect DAP in samples from Human, Rat and Mouse. It has been validated for ELISA, WB, IF and IHC (p).

Quick Overview for DAP antibody (C-Term) (ABIN6991874)

Target: DAP (Death-Associated Protein (DAP))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAP antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DAP Antibody

Binding Specificity: C-Term

Specificity: DAP antibody is human, mouse and rat reactive.

Purification: DAP Antibody is affinity chromatography purified via peptide column.

Immunogen: DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP. The immunogen is located within the last 50 amino acids of DAP.

Isotype: IgG

Application Details

Application Notes: DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DAP Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for DAP

Target: DAP (Death-Associated Protein (DAP))

Alternative Name: DAP

Background: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15 kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51, under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).

Molecular Weight: Predicted: 11 kDa

Observed: 11 kDa

Gene ID: 1611

NCBI Accession: NP_004385

UniProt: P51397

Pathways: Positive Regulation of Endopeptidase Activity, Feeding Behaviour