DAP Antibody is affinity chromatography purified via peptide column.
Immunogen
DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP. The immunogen is located within the last 50 amino acids of DAP.
DAP
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
DAP Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
DAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Rap7a antibody, 4921531N22Rik antibody, AI789753 antibody, AU040360 antibody, MGC53808 antibody, MGC85522 antibody, DAP1 antibody, DAP antibody, Dap antibody, ACYPI007535 antibody, DAP-1 antibody, dap1a antibody, wu:fa16h08 antibody, wu:fc06d11 antibody, death associated protein antibody, death-associated protein antibody, death-associated protein S homeolog antibody, DAP antibody, Dap antibody, dap.S antibody, dap antibody
Background
DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15 kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51, under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).