FLI1 antibody (C-Term)

Catalog No. ABIN6991893

The Rabbit Polyclonal anti-FLI1 antibody (ABIN6991893) specifically detects FLI1 in WB, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FLI1 antibody (C-Term) (ABIN6991893)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-FLI1 Antibody

Binding Specificity: C-Term

Specificity: FLI1 antibody is human, mouse and rat reactive. At least four isoforms of FLI1 are known to exist, this antibody will detect all four.

Purification: FLI1 Antibody is affinity chromatography purified via peptide column.

Immunogen: FLI1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human FLI1. The immunogen is located within the last 50 amino acids of FLI1 .

Isotype: IgG

Application Details

Application Notes: FLI1 antibody can be used for detection of FLI1 by Western blot at 0.5 - 1 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: FLI1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: FLI1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: FLI1

Background: The Friend leukemia virus integration 1 (FLI1) protein is a transcription factor containing an ETS DNA-binding domain and is highly expressed in hematopoietic cell lineages and vascular endothelial cells (1). FLI1 plays important roles in megakaryocytic differentiation (2) and vascular homeostasis (3). The FLI1 gene can undergo a t(11,22)(q24,q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. (4). Abnormal expression of FLI1 can be used as an adverse prognostic indicator for acute myeloid leukemia (5).

Molecular Weight: Predicted: 50 kDa

Observed: 52kDa

Gene ID: 2313

NCBI Accession: NP_002008

UniProt: Q01543