IL12A antibody (C-Term)

Catalog No. ABIN6991911

This anti-IL12A antibody is a Rabbit Polyclonal antibody detecting IL12A in WB, ELISA, IHC (p) and IF. Suitable for Human.

Quick Overview for IL12A antibody (C-Term) (ABIN6991911)

Target: IL12A (Interleukin 12 alpha (IL12A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL12A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-IL12A Antibody

Binding Specificity: C-Term

Specificity: IL-12A antibody is human specific. IL-12A antibody will not cross-react with IL-12B.

Purification: IL-12A antibody is affinity chromatography purified via peptide column.

Immunogen: IL-12A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IL-12A. The immunogen is located within the last 50 amino acids of IL-12A.

Isotype: IgG

Application Details

Application Notes: IL-12A antibody can be used for detection of IL-12A by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IL-12A antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IL-12A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: IL-12A

Background: IL-12A is subunit of the cytokine IL-12 which acts on T- and natural killer cells, and has a broad array of biological activities. IL-12 is a disulfide-linked heterodimer composed of the 35-kD subunit and a 40-kD subunit that is a member of the cytokine receptor family (1). IL-12 is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells (1,2). The responses of lymphocytes to IL-12 are mediated by the activator of transcription protein STAT4 (2). Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity (3).

Molecular Weight: Predicted: 22 kDa

Observed: 22 kDa

Gene ID: 3592

NCBI Accession: NP_000873

UniProt: P29459

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation