BHLHA15 antibody (AA 60-110)

Catalog No. ABIN6991922

The Rabbit Polyclonal anti-BHLHA15 antibody has been validated for ELISA, WB, IF and IHC (p). It is suitable to detect BHLHA15 in samples from Human, Mouse and Rat.

Quick Overview for BHLHA15 antibody (AA 60-110) (ABIN6991922)

Target: BHLHA15 (Basic Helix-Loop-Helix Family, Member A15 (BHLHA15))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BHLHA15 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BHLHA15 Antibody

Binding Specificity: AA 60-110

Specificity: BHLHA15 antibody is human, mouse and rat reactive.

Purification: BHLHA15 antibody is affinity chromatography purified via peptide column.

Immunogen: BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15. The immunogen is located within amino acids 60 - 110 of BHLHA15.

Isotype: IgG

Application Details

Application Notes: BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: BHLHA15 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: BHLHA15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for BHLHA15

Target: BHLHA15 (Basic Helix-Loop-Helix Family, Member A15 (BHLHA15))

Alternative Name: BHLHA15

Background: BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).

Molecular Weight: Predicted: 21 kDa

Observed: 24 kDa

Gene ID: 168620

NCBI Accession: NP_803238

UniProt: Q7RTS1