MAP1LC3A antibody (N-Term)

Catalog No. ABIN6991931

The Rabbit Polyclonal anti-MAP1LC3A antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect MAP1LC3A in samples from Human.

Quick Overview for MAP1LC3A antibody (N-Term) (ABIN6991931)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAP1LC3A Antibody

Binding Specificity: N-Term

Specificity: MAP1LC3A antibody is human specific. At least two isoforms of MAP1LC3A are known to exist. MAP1LC3A antibody is predicted to not cross-react with MAP1LC3B or MAP1LC3C.

Purification: MAP1LC3A antibody is affinity chromatography purified via peptide column.

Immunogen: MAP1LC3A antibody was raised against a 10 amino acid peptide near the amino terminus of human MAP1LC3A. The immunogen is located within the first 50 amino acids of MAP1LC3A.

Isotype: IgG

Application Details

Application Notes: MAP1LC3A antibody can be used for detection of MAP1LC3A by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MAP1LC3A antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MAP1LC3A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: MAP1LC3A

Background: Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3A belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). MAP1LC3A is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3A, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3).

Molecular Weight: Predicted: 14 kDa

Observed: 17 kDa

Gene ID: 84557

NCBI Accession: NP_852610

UniProt: Q9H492

Pathways: Autophagy