ULK3 antibody (AA 230-280)

Catalog No. ABIN6991934

The Rabbit Polyclonal anti-ULK3 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect ULK3 in samples from Human.

Quick Overview for ULK3 antibody (AA 230-280) (ABIN6991934)

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ULK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-ULK3 Antibody

Binding Specificity: AA 230-280

Specificity: ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.

Purification: ULK3 antibody is affinity chromatography purified via peptide column.

Immunogen: ULK3 antibody was raised against a 19 amino acid peptide near the center of human ULK3. The immunogen is located within amino acids 230 - 280 of ULK3.

Isotype: IgG

Application Details

Application Notes: ULK3 antibody can be used for detection of ULK3 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ULK3 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ULK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for ULK3

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Alternative Name: ULK3

Background: ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3). ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4). Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins, this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5).

Molecular Weight: Predicted: 52 kDa

Observed: 52 kDa

Gene ID: 25989

NCBI Accession: NP_001092906

UniProt: Q6PHR2