alpha Tubulin antibody (N-Term)

Catalog No. ABIN6991939

This Rabbit Polyclonal antibody specifically detects alpha Tubulin in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for alpha Tubulin antibody (N-Term) (ABIN6991939)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-alpha Tubulin Antibody

Binding Specificity: N-Term

Specificity: Alpha-tubulin antibody is human, mouse and rat reactive.

Purification: Alpha-tubulin antibody is affinity chromatography purified via peptide column.

Immunogen: Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin. The immunogen is located within the first 50 amino acids of Alpha-tubulin.

Isotype: IgG

Application Details

Application Notes: Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Alpha-tubulin antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Alpha-tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Alpha-tubulin

Background: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

Molecular Weight: Predicted: 50 kDa

Observed: 45 kDa

Gene ID: 7278

NCBI Accession: NP_005992

UniProt: Q13748

Pathways: Microtubule Dynamics