SPNS1/Spinster 1 antibody (N-Term)

Catalog No. ABIN6991957

Product Details anti-SPNS1/Spinster 1 Antibody

Target: SPNS1/Spinster 1 (SPNS1) (Spinster Homolog 1 (SPNS1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPNS1/Spinster 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: SPINSTER antibody is human specific. At least four isoforms of SPINSTER are known to exist. This antibody is predicted to not cross-react with other members of the spinster family of proteins.

Purification: SPINSTER antibody is affinity chromatography purified via peptide column.

Immunogen: SPINSTER antibody was raised against an 18 amino acid peptide near the amino terminus of human SPINSTER. The immunogen is located within the first 50 amino acids of SPINSTER.

Isotype: IgG

Application Details

Application Notes: SPINSTER antibody can be used for detection of SPINSTER by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SPINSTER antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SPINSTER antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for SPNS1/Spinster 1

Target: SPNS1/Spinster 1 (SPNS1) (Spinster Homolog 1 (SPNS1))

Alternative Name: SPINSTE (SPNS1 Products)

Synonyms: etID64740.3 antibody, nrs antibody, spinl antibody, wu:fb95b12 antibody, wu:fi37e11 antibody, 2210013K02Rik antibody, Spin1 antibody, Spinl antibody, HSpin1 antibody, LAT antibody, PP2030 antibody, SPIN1 antibody, SPINL antibody, spinster antibody, RGD1305613 antibody, sphingolipid transporter 1 (putative) antibody, spinster homolog 1 (Drosophila) antibody, spinster homolog 1 antibody, spinster homolog 1 L homeolog antibody, sphingolipid transporter 1 antibody, SPNS1 antibody, spns1 antibody, Spns1 antibody, spns1.L antibody

Background: SPINSTER, also known as SPNS1 or SPIN1, is a 528 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and belongs to the spinster subfamily of the major facilitator superfamily (1). SPINSTER interacts with Bcl-x and Bcl-2 and, via this interaction, is thought to be involved in necrotic or autophagic cell death (2). The related protein SPNS2 is critical for the normal lymphocyte localization and mammalian immune system function (1,3).

Molecular Weight: Predicted: 58 kDa

Observed: 56 kDa

Gene ID: 83985

NCBI Accession: NP_001135920

UniProt: Q9H2V7