UCP1 antibody (C-Term)

Catalog No. ABIN6991968

The Rabbit Polyclonal anti-UCP1 antibody (ABIN6991968) specifically detects UCP1 in WB, ELISA and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UCP1 antibody (C-Term) (ABIN6991968)

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UCP1 Antibody

Binding Specificity: C-Term

Specificity: UCP1 antibody is human, mouse and rat reactive.

Purification: UCP1 antibody is affinity chromatography purified via peptide column.

Immunogen: UCP1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human UCP1 (GenBank accession. The immunogen is located within the last 50 amino acids of UCP1.

Isotype: IgG

Application Details

Application Notes: UCP1 antibody can be used for detection of UCP1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 2.5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: UCP1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: UCP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: UCP1

Background: The mitochondrial brown fat uncoupling protein 1 (UCP1) is a member of the family of mitochondrial anion carrier proteins (MACP) (1). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells (1). UCP1 is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat (1).

Molecular Weight: Predicted: 34 kDa

Observed: 35 kDa

Gene ID: 7350

NCBI Accession: NP_068605

UniProt: P25874

Pathways: Proton Transport, Brown Fat Cell Differentiation