ME1 antibody (N-Term)

Catalog No. ABIN6991979

The Rabbit Polyclonal anti-ME1 antibody is suitable to detect ME1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC (p).

Quick Overview for ME1 antibody (N-Term) (ABIN6991979)

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ME1 Antibody

Binding Specificity: N-Term

Specificity: ME1 antibody is human, mouse and rat reactive. ME1 antibody is predicted not to cross-react with ME2.

Purification: ME1 antibody is affinity chromatography purified via peptide column.

Immunogen: ME1 antibody was raised against a 16 amino acid peptide near the amino terminus of human ME1. The immunogen is located within the first 50 amino acids of ME1.

Isotype: IgG

Application Details

Application Notes: ME1 antibody can be used for detection of ME1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ME1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ME1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Alternative Name: ME1

Background: ME1, also known as NADP-ME, MES or HUMNDME, may play an important role as a housekeeping protein within the cell (1). ME1 is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family (2). It is expressed ubiquitously with highest expression in liver and white adipose tissue. ME1 functions as an NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (3). The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles (3,4). ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet (5).

Molecular Weight: Predicted: 63 kDa

Observed: 63 kDa

Gene ID: 4199

NCBI Accession: NP_002386

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha