WAPAL antibody (N-Term)

Catalog No. ABIN6992007

This anti-WAPAL antibody is a Rabbit Polyclonal antibody detecting WAPAL in ELISA, WB, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for WAPAL antibody (N-Term) (ABIN6992007)

Target: WAPAL (Wings Apart-Like Protein Homolog (WAPAL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WAPAL antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-WAPAL Antibody

Binding Specificity: AA 30-80, N-Term

Specificity: WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist.

Purification: WAPL antibody is affinity chromatography purified via peptide column.

Immunogen: WAPL antibody was raised against an 18 amino acid peptide near the amino terminus of human WAPL. The immunogen is located within amino acids 30 - 80 of WAPL.

Isotype: IgG

Application Details

Application Notes: WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: WAPL antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: WAPL antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for WAPAL

Target: WAPAL (Wings Apart-Like Protein Homolog (WAPAL))

Alternative Name: WAPL

Background: WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth (1). It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1) (1,2,4). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits (3,4). WAPL is a new regulator of the development and metastasis of cancerous tissue (4,5).

Molecular Weight: Predicted: 131 kDa

Observed: 140 kDa

Gene ID: 23063

NCBI Accession: NP_055860

UniProt: Q7Z5K2

Pathways: Chromatin Binding