ATG4C antibody (C-Term)

Catalog No. ABIN6992038

This Rabbit Polyclonal antibody specifically detects ATG4C in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATG4C antibody (C-Term) (ABIN6992038)

Target: ATG4C (Autophagy related 4C Cysteine Peptidase (ATG4C))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG4C Antibody

Binding Specificity: C-Term

Specificity: ATG4C antibody is human, mouse and rat reactive. ATG4C is predicted to not cross-react with other ATG4 proteins.

Purification: ATG4C antibody is affinity chromatography purified via peptide column.

Immunogen: ATG4C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4C. The immunogen is located within the last 50 amino acids of ATG4C.

Isotype: IgG

Application Details

Application Notes: ATG4C antibody can be used for detection of ATG4C by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ATG4C antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ATG4C antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for ATG4C

Target: ATG4C (Autophagy related 4C Cysteine Peptidase (ATG4C))

Alternative Name: ATG4C

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4C, also known as autophagin-3, is one of four mammalian orthologs of the yeast ATG4 protein, all four are cysteine proteases (3). Recent studies have shown that ATG4C and Beclin-1 are regulated by the microRNA miR-376b, overexpression of miR-376b led to decreased mRNA and protein levels, thereby blocking starvation and TOR inhibition-related autophagy (4).

Molecular Weight: Predicted: 50 kDa

Observed: 57 kDa

Gene ID: 84938

NCBI Accession: NP_835739

UniProt: Q96DT6

Pathways: Autophagy