NOX1 antibody (AA 210-260)

Catalog No. ABIN6992042

The Rabbit Polyclonal anti-NOX1 antibody is suitable to detect NOX1 in samples from Human. It has been validated for WB, ELISA, IHC (p) and IF.

Quick Overview for NOX1 antibody (AA 210-260) (ABIN6992042)

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-NOX1 Antibody

Binding Specificity: AA 210-260

Specificity: NOX1 antibody is human specific.

Purification: NOX1 antibody is affinity chromatography purified via peptide column.

Immunogen: NOX1 antibody was raised against a 15 amino acid peptide near the center of human NOX1. The immunogen is located within amino acids 210 - 260 of NOX1.

Isotype: IgG

Application Details

Application Notes: NOX1 antibody can be used for detection of NOX1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NOX1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NOX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for NOX1

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Alternative Name: NOX1

Background: Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress (1). NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox (1). Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv (2). NOH-1S is a voltage-gated proton channel that participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain (1-3). NOX1 have the potential to be effective treatments for a range of ischemic diseases (4).

Molecular Weight: Predicted: 62 kDa

Observed: 64 kDa

Gene ID: 27035

NCBI Accession: NP_008983

UniProt: Q9Y5S8

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport