NOX1 antibody (Internal Region)

Catalog No. ABIN768583

Product Details anti-NOX1 Antibody

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: NOX1

Sequence: DKATDIVTGL KQK

Specificity: This antibody is expected to recognize both reported isoforms (NP_008983.2, NP_39249.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DKATDIVTGLKQK, from the internal region of the protein sequence according to NP_008983.2, NP_39249.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70-72 kDa band observed in lysates of cell lines A549, CaCo-2, HeLa and U937, (calculated MW of 64.9 kDa according to NP_008983.2,). This molecular weight is routinely observed by other sources and was successfully blocked by incubatio

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NOX1

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Alternative Name: NOX1 (NOX1 Products)

Background: NOX1, NADPH oxidase 1, GP91-2, MOX1, NOH-1, NOH1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NADPH oxidase 1 variant NOH-1L, NADPH oxidase homolog-1, NOX-1, mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating), mitogenic oxida

Molecular Weight: 64.9kDa according to NP_008983.2

Gene ID: 27035, 237038

NCBI Accession: NP_008983, NP_39249

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport