NOX3 antibody (N-Term)

Catalog No. ABIN6992044

This Rabbit Polyclonal antibody specifically detects NOX3 in ELISA, WB, IHC (p) and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for NOX3 antibody (N-Term) (ABIN6992044)

Target: NOX3 (NADPH Oxidase 3 (NOX3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOX3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-NOX3 Antibody

Binding Specificity: AA 110-160, N-Term

Specificity: NOX3 antibody is human and mouse reactive. NOX3 is predicted to not cross-react with other NOX proteins.

Purification: NOX3 antibody is affinity chromatography purified via peptide column.

Immunogen: NOX3 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX3. The immunogen is located within amino acids 110 - 160 of NOX3.

Isotype: IgG

Application Details

Application Notes: NOX3 antibody can be used for detection of NOX3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NOX3 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NOX3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for NOX3

Target: NOX3 (NADPH Oxidase 3 (NOX3))

Alternative Name: NOX3

Background: The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX3 is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith (2,3). It has been suggested that NOX3 is activated by the Transient Receptor Potential Vanilloid 1 (TRPV1), and this activity causes increased levels of reactive oxygen species in the inner ear, which in turn leads to STAT1-mediated inflammation and hearing loss (4).

Molecular Weight: Predicted: 62 kDa

Observed: 72 kDa

Gene ID: 50508

NCBI Accession: NP_056533

UniProt: Q9HBY0