E2F3 antibody (AA 150-200)

Catalog No. ABIN6992046

This anti-E2F3 antibody is a Rabbit Polyclonal antibody detecting E2F3 in WB, ELISA, IHC (p) and IF. Suitable for Human and Mouse.

Quick Overview for E2F3 antibody (AA 150-200) (ABIN6992046)

Target: E2F3

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-E2F3 Antibody

Binding Specificity: AA 150-200

Specificity: E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist, this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.

Purification: E2F3 antibody is affinity chromatography purified via peptide column.

Immunogen: E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3. The immunogen is located within amino acids 150 - 200 of E2F3.

Isotype: IgG

Application Details

Application Notes: E2F3 antibody can be used for detection of E2F3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: E2F3 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: E2F3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for E2F3

Target: E2F3

Background: The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins. E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1). Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2). Altered copy number and activity of this gene have been observed in a number of human cancers (3).

Molecular Weight: Predicted: 51 kDa

Observed: 56 kDa

Gene ID: 1871

NCBI Accession: NP_001940

UniProt: O00716