PIK3R4 antibody (C-Term)

Catalog No. ABIN6992056

This Rabbit Polyclonal antibody specifically detects PIK3R4 in ELISA, WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PIK3R4 antibody (C-Term) (ABIN6992056)

Target: PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PIK3R4 Antibody

Binding Specificity: AA 1290-1340, C-Term

Specificity: PIK3R4 antibody is human, mouse and rat reactive.

Purification: PIK3R4 antibody is affinity chromatography purified via peptide column.

Immunogen: PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4. The immunogen is located within amino acids 1290 - 1340 of PIK3R4.

Isotype: IgG

Application Details

Application Notes: PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PIK3R4 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PIK3R4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for PIK3R4

Target: PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))

Alternative Name: PIK3R4

Background: PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1,2). PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1). PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5).

Molecular Weight: Predicted: 149 kDa

Observed: 140 kDa

Gene ID: 30849

NCBI Accession: NP_055417

UniProt: Q99570