LC3B antibody (AA 20-70)

Catalog No. ABIN6992057

The Rabbit Polyclonal anti-LC3B antibody is suitable to detect LC3B in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC (p).

Quick Overview for LC3B antibody (AA 20-70) (ABIN6992057)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LC3B Antibody

Binding Specificity: AA 20-70

Specificity: MAP1LC3 antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3 are known to exist. MAP1LC3 antibody is predicted to detect MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Purification: MAP1LC3 antibody is affinity chromatography purified via peptide column.

Immunogen: MAP1LC3 antibody was raised against a 12 amino acid peptide near the center of human MAP1LC3A. The immunogen is located within amino acids 20 - 70 of MAP1LC3.

Isotype: IgG

Application Details

Application Notes: MAP1LC3 antibody can be used for detection of MAP1LC3 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MAP1LC3 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MAP1LC3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: MAP1LC3

Background: Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3 is a subfamily of three related proteins belonging to the MAP1 LC3 family and it includes MAP1LC3A, MAP1LC3B, and MAP1LC3C (2). MAP1LC3 is the mammalian homolog of yeast ATG8 and is essential for autophagy and associated with the autophagosome membranes after processing (3). The three isoforms exhibit distinct expression patterns and both MAP1LC3A and MAP1LC3B but not MAP1LC3B, are post-translationally modified, suggesting the three isoforms may have different physiological functions (4).

Molecular Weight: Predicted: 13 kDa

Observed: 18 kDa

Gene ID: 84557

NCBI Accession: NP_115903

UniProt: Q9H492

Pathways: Autophagy