VEZT antibody (AA 480-530)

Catalog No. ABIN6992059

This Rabbit Polyclonal antibody specifically detects VEZT in ELISA, WB, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for VEZT antibody (AA 480-530) (ABIN6992059)

Target: VEZT (Vezatin, Adherens Junctions Transmembrane Protein (VEZT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEZT antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-VEZT Antibody

Binding Specificity: AA 480-530

Specificity: VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist.

Purification: VEZT antibody is affinity chromatography purified via peptide column.

Immunogen: VEZT antibody was raised against a 17 amino acid peptide near the center of human VEZT. The immunogen is located within amino acids 480 - 530 of VEZT.

Isotype: IgG

Application Details

Application Notes: VEZT antibody can be used for detection of VEZT by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: VEZT antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: VEZT antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for VEZT

Target: VEZT (Vezatin, Adherens Junctions Transmembrane Protein (VEZT))

Alternative Name: VEZT

Background: Vezatin (VEZT) is a transmembrane protein which localizes to adherens junctions and binds to myosin VIIA and the cadherin-catenins complex (1). Expression of this gene in gastric cancer tissues is decreased due to hypermethylation of the promoter. Expression of this gene may also be inhibited by binding of a specific microRNA to a target sequence in the 3' UTR of the transcripts (2). VEZT has also been shown to be required for morphogenesis of the preimplantation mouse embryo (3).

Molecular Weight: Predicted: 63, 68, 78, 81, 83, 86 kDa

Observed: 60 kDa

Gene ID: 55591

NCBI Accession: NP_060069

UniProt: Q9HBM0