WWC1 antibody (N-Term)

Catalog No. ABIN6992072

Product Details anti-WWC1 Antibody

Target: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Binding Specificity: AA 60-110, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WWC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: WWC1 antibody is human, mouse and rat reactive. At least three isoforms of WWC1 are known to exist, this antibody will detect all three isoforms.

Purification: WWC1 antibody is affinity chromatography purified via peptide column.

Immunogen: WWC1 antibody was raised against an 18 amino acid peptide near the amino terminus of human WWC1. The immunogen is located within amino acids 60 - 110 of WWC1.

Isotype: IgG

Application Details

Application Notes: WWC1 antibody can be used for detection of WWC1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: WWC1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: WWC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for WWC1

Target: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Alternative Name: WWC1 (WWC1 Products)

Synonyms: CG12600 antibody, CG33967 antibody, CG7552 antibody, Dmel\\CG33967 antibody, Kibra antibody, ORE-2 antibody, RE26350 antibody, kib antibody, l(3)02404 antibody, l(3)j5C3 antibody, l(3)neo42 antibody, HBEBP3 antibody, HBEBP36 antibody, KIBRA antibody, AA408228 antibody, AU017197 antibody, BC037006 antibody, mKIAA0869 antibody, CG33967 gene product from transcript CG33967-RB antibody, WW and C2 domain containing 1 antibody, WW, C2 and coiled-coil domain containing 1 antibody, kibra antibody, WWC1 antibody, Wwc1 antibody

Background: The WW and C2 domain containing 1 (WWC1) protein, also known as KIBRA, possesses two WW domains and an internal C2-like domain (1). WWC1 was originally identified as a memory performance-associated protein in humans (2) and has recently been shown to be a novel regulator of the Hippo pathway (3). WWC1 is phosphorylated by the mitotic kinases Aurora-A and -B (4), and in turn activates the Aurora kinases and is required for precise chromosome alignment during mitosis (5).

Molecular Weight: Predicted: 123 kDa

Observed: 110 kDa

Gene ID: 23286

NCBI Accession: NP_001155133

UniProt: Q8IX03