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LIMA1 antibody (N-Term)

LIMA1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6992089
  • Target See all LIMA1 Antibodies
    LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))
    Binding Specificity
    • 9
    • 8
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 230-280, N-Term
    Reactivity
    • 36
    • 9
    • 7
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 30
    • 6
    Rabbit
    Clonality
    • 30
    • 6
    Polyclonal
    Conjugate
    • 26
    • 2
    • 2
    • 2
    • 2
    • 2
    This LIMA1 antibody is un-conjugated
    Application
    • 35
    • 18
    • 8
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist, this antibody will only detect the three largest isoforms.
    Purification
    LIMA1 antibody is affinity chromatography purified via peptide column.
    Immunogen
    LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1. The immunogen is located within amino acids 230 - 280 of LIMA1.
    Isotype
    IgG
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    Discover our top product LIMA1 Primary Antibody
  • Application Notes
    LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    LIMA1 antibody is supplied in PBS containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C,4 °C
    Storage Comment
    LIMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
  • Target
    LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))
    Alternative Name
    LIMA1 (LIMA1 Products)
    Synonyms
    eplin antibody, LIMA1 antibody, EPLIN antibody, MGC146397 antibody, SREBP3 antibody, 1110021C24Rik antibody, 3526402A12Rik antibody, D15Ertd366e antibody, Eplin antibody, LIM domain and actin binding 1 antibody, Lima1 antibody, LIMA1 antibody, lima1 antibody
    Background
    The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).
    Molecular Weight
    Predicted: 50, 66, 84 kDa

    Observed: 65 kDa
    Gene ID
    51474
    NCBI Accession
    NP_001107018
    UniProt
    Q9UHB6
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