HES5 antibody (N-Term)

Catalog No. ABIN6992090

Product Details anti-HES5 Antibody

Target: HES5 (Hairy and Enhancer of Split 5 (HES5))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HES5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist, this antibody will detect both isoforms.

Purification: HES5 antibody is affinity chromatography purified via peptide column.

Immunogen: HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5. The immunogen is located within the first 50 amino acids of HES5.

Isotype: IgG

Application Details

Application Notes: HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: HES5 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: HES5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for HES5

Target: HES5 (Hairy and Enhancer of Split 5 (HES5))

Alternative Name: HES5 (HES5 Products)

Synonyms: fk51a02 antibody, her15 antibody, hes5 antibody, wu:fk51a02 antibody, zgc:136520 antibody, bHLHb38 antibody, hairy and enhancer of split-related 15, tandem duplicate 1 antibody, hes family bHLH transcription factor 5 antibody, hairy and enhancer of split 5 (Drosophila) antibody, transcription factor HES-5-like antibody, her15.1 antibody, HES5 antibody, hes5 antibody, LOC100221262 antibody, LOC100226434 antibody, Hes5 antibody

Background: HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).

Molecular Weight: Predicted: 18 kDa

Observed: 17 kDa

Gene ID: 388585

NCBI Accession: NP_001010926

UniProt: Q5TA89

Pathways: Stem Cell Maintenance, Tube Formation