ATG4B antibody (C-Term)

Catalog No. ABIN6992096

The Rabbit Polyclonal anti-ATG4B antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect ATG4B in samples from Human, Mouse and Rat.

Quick Overview for ATG4B antibody (C-Term) (ABIN6992096)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-ATG4B Antibody

Binding Specificity: C-Term

Specificity: ATG4B antibody is human, mouse and rat reactive. At least two isoforms of ATG4B are known to exist, this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.

Purification: ATG4B antibody is affinity chromatography purified via peptide column.

Immunogen: ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B. The immunogen is located within the last 50 amino acids of ATG4B.

Isotype: IgG

Application Details

Application Notes: ATG4B antibody can be used for detection of ATG4B by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ATG4B antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ATG4B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Alternative Name: ATG4B

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4B, also known as AUTL1, is one of four mammalian orthologs of the yeast ATG4 protein, all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4). ATG4B has been found to be a novel protective protein in inflammatory colitis (5).

Molecular Weight: Predicted: 43 kDa

Observed: 46 kDa

Gene ID: 23192

NCBI Accession: NP_037457

UniProt: Q9Y4P1

Pathways: Autophagy