ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist, this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A.
Purification
ADORA2B antibody is affinity chromatography purified via peptide column.
Immunogen
ADORA2B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADORA2B. The immunogen is located within the last 50 amino acids of ADORA2B.
ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
ADORA2B antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
ADORA2B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2). Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5). Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).