WSB1 antibody (N-Term)

Catalog No. ABIN6992130

The Rabbit Polyclonal anti-WSB1 antibody (ABIN6992130) specifically detects WSB1 in WB, ELISA, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for WSB1 antibody (N-Term) (ABIN6992130)

Target: WSB1 (WD Repeat and SOCS Box Containing 1 (WSB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WSB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-WSB1 Antibody

Binding Specificity: AA 70-120, N-Term

Specificity: WSB1 antibody is human specific.

Purification: WSB1 antibody is affinity chromatography purified via peptide column.

Immunogen: WSB1 antibody was raised against a 16 amino acid peptide near the amino terminus of human WSB1. The immunogen is located within amino acids 70 - 120 of WSB1.

Isotype: IgG

Application Details

Application Notes: WSB1 antibody can be used for detection of WSB1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: WSB1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: WSB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for WSB1

Target: WSB1 (WD Repeat and SOCS Box Containing 1 (WSB1))

Alternative Name: WSB1

Background: The WD repeat and SOCS box-containing 1 (WSB1, also known as SWIP1), is a member of the WD-protein subfamily (1). It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus and expressed as multiple alternatively spliced isoforms (1,2). WSB1 is thought to function as part of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex that mediates the ubiquitination and proteasomal degradation of target proteins, such as DIO2 (3). Overexpression of WSB1 is implicated in pancreatic cancer progression, suggesting a role for WSB1 in carcinogenesis (4,5).

Molecular Weight: Predicted: 46 kDa

Observed: 46 kDa

Gene ID: 26118

NCBI Accession: NP_056441

UniProt: Q9Y6I7