UBD antibody (AA 80-130)

Catalog No. ABIN6992138

The Rabbit Polyclonal anti-UBD antibody is suitable to detect UBD in samples from Human. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for UBD antibody (AA 80-130) (ABIN6992138)

Target: UBD (Ubiquitin D (UBD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBD Antibody

Binding Specificity: AA 80-130

Specificity: UBD antibody is human specific.

Purification: UBD antibody is affinity chromatography purified via peptide column.

Immunogen: UBD antibody was raised against a 16 amino acid peptide near the center of human UBD. The immunogen is located within amino acids 80 - 130 of UBD.

Isotype: IgG

Application Details

Application Notes: UBD antibody can be used for detection of UBD by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: UBD antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: UBD antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for UBD

Target: UBD (Ubiquitin D (UBD))

Alternative Name: UBD

Background: UBD, also designated ubiquitin D or diubiquitin, plays a role in antigen presentation, cytokine response and apoptosis. It is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin (1,2). UBD is inducible by interferon gamma and TNF-alpha and regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha (3). It may also regulate cell growth during dendritic cell or B cell activation and development (4,5).

Molecular Weight: Predicted: 18 kDa

Observed: 17 kDa

Gene ID: 10537

NCBI Accession: NP_006389

UniProt: O15205

Pathways: Ubiquitin Proteasome Pathway