Hexokinase 1 antibody (C-Term)

Catalog No. ABIN6992150

The Rabbit Polyclonal anti-Hexokinase 1 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect Hexokinase 1 in samples from Human, Mouse and Rat.

Quick Overview for Hexokinase 1 antibody (C-Term) (ABIN6992150)

Target: Hexokinase 1 (HK1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Hexokinase 1 Antibody

Binding Specificity: AA 830-880, C-Term

Specificity: Hexokinase 1 antibody is human, mouse and rat reactive. Multiple isoforms of Hexokinase 1 are known to exist.

Purification: Hexokinase 1 antibody is affinity chromatography purified via peptide column.

Immunogen: Hexokinase 1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Hexokinase 1. The immunogen is located within amino acids 830 - 880 of Hexokinase 1.

Isotype: IgG

Application Details

Application Notes: Hexokinase 1 antibody can be used for detection of Hexokinase 1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Hexokinase 1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Hexokinase 1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: Hexokinase 1

Background: There are four major glucose-phosphorylating isoenzymes, designated Hexokinase 1 I, II, III, and IV (1). Hexokinase 1 activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway (1,2). Hexokinase 1 2 is the predominant Hexokinase 1 isozyme expressed in insulin-responsive tissues such as skeletal muscle and its expression is insulin-responsive (3). It is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (4).

Molecular Weight: Predicted: 101 kDa

Observed: 103 kDa

Gene ID: 3098

NCBI Accession: NP_000179

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effect