LSD1 antibody (N-Term)

Catalog No. ABIN6992152

This Rabbit Polyclonal antibody specifically detects LSD1 in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LSD1 antibody (N-Term) (ABIN6992152)

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LSD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LSD1 Antibody

Binding Specificity: AA 90-140, N-Term

Specificity: At least two isoforms of LSD1 are known to exist, this antibody detects both.

Purification: LSD1 antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-LSD1 antibody was raised against a peptide corresponding to 19 amino acids near the amino terminus of human LSD1. The immunogen is located within amino acids 90-140 of LSD1.

Isotype: IgG

Application Details

Application Notes: WB: 1 - 2 μ,g/mL, IHC-P: 5 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LSD1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LSD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for LSD1

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Alternative Name: LSD1

Background: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.

Molecular Weight: Predicted: 93kD

Observed: 93 kD kDa

Gene ID: 23028

NCBI Accession: NP_001009999

UniProt: O60341

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effect