CD80 antibody (Extracellular Domain)

Catalog No. ABIN6992239

This Mouse Monoclonal antibody specifically detects CD80 in FACS, ELISA, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for CD80 antibody (Extracellular Domain) (ABIN6992239)

Target: CD80

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD80 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: 8G12

Product Details anti-CD80 Antibody

Binding Specificity: Extracellular Domain

Purification: CD80 Antibody is supplied as protein A purified IgG2b.

Immunogen: CD80 antibody was raised against the extracellular domain of human CD80.

Isotype: IgG2b

Application Details

Application Notes: CD80 antibody can be used for immunohistochemistry starting at 2 - 5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL. Flow cytometry at 10 μ,g/mL.

Antibody validated: Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CD80 Antibody is supplied in PBS containing 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CD80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background: CD80 Antibody: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).

Molecular Weight: Predicted: 32 kDa

Observed: 50 kDa

Gene ID: 941

NCBI Accession: NP_005182

UniProt: P33681

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints