LAG3 antibody (Extracellular Domain)

Catalog No. ABIN6992260

Product Details anti-LAG3 Antibody

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: LAG-3 Antibody is supplied as protein A purified IgG1.

Immunogen: LAG-3 antibody was raised against the extracellular domain of human LAG-3

Clone: 2G8

Isotype: IgG1 kappa

Application Details

Application Notes: LAG-3 antibody can be used for ELISA starting at 0.25 μ,g/mL. For Western blot start at 0.25 μ,g/mL. For Immunocytochemistry start at 1 μ,g/mL. For Immunofluorescence start at 10 μ,g/mL. For Immunohistochemistry start at 5 μ,g/mL. For Flow Cytometry start at 1 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LAG-3 Antibody is supplied in PBS containing 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LAG-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG3 (LAG3 Products)

Synonyms: CD223 antibody, LAG-3 antibody, Ly66 antibody, lymphocyte activating 3 antibody, lymphocyte-activation gene 3 antibody, LAG3 antibody, Lag3 antibody

Background: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

Molecular Weight: Predicted: 55 kDa

Observed: 60, 70, 80 kDa

Gene ID: 3902

NCBI Accession: NP_002277

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints