LAG3 antibody (Extracellular Domain)

Catalog No. ABIN6992261

The Mouse Monoclonal anti-LAG3 antibody has been validated for ELISA, ICC and FACS. It is suitable to detect LAG3 in samples from Human.

Quick Overview for LAG3 antibody (Extracellular Domain) (ABIN6992261)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 7A7

Product Details anti-LAG3 Antibody

Binding Specificity: Extracellular Domain

Purification: LAG-3 Antibody is supplied as protein A purified IgG2a.

Immunogen: LAG-3 antibody was raised against the extracellular domain of human LAG-3

Isotype: IgG2a kappa

Application Details

Application Notes: LAG-3 antibody can be used for ELISA starting at 0.25 μ,g/mL. For Immunocytochemistry start at 1 μ,g/mL. For Flow Cytometry start at 1 μ,g/mL.

Antibody validated: Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LAG-3 Antibody is supplied in PBS containing 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LAG-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG3

Background: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

Gene ID: 3902

NCBI Accession: NP_002277

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints