SARS-CoV-2 NSP12 (RdRP) antibody (C-Term)

Catalog No. ABIN6992285

Product Details anti-SARS-CoV-2 NSP12 (RdRP) Antibody

Target: SARS-CoV-2 NSP12 (RdRP) (SARS-CoV-2 RdRP) (SARS-CoV-2 RNA-dependent RNA Polymerase (NSP12) (SARS-CoV-2 RdRP))

Binding Specificity: C-Term

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 NSP12 (RdRP) antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: SARS-CoV-2 (COVID-19) NSP12 antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-SARS-CoV-2 (COVID-19) NSP12 antibody was raised against a peptide corresponding to 16 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) NSP12 protein. The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) NSP12 protein.

Isotype: IgG

Application Details

Application Notes: WB: 1-2 μ,g/mL

Antibody validated: SARS-CoV-2 (COVID-19) NSP12 antibody can detect 2 ng of free peptide at 1 ?g/mL in ELISA. It can detect SARS-CoV-2 NSP12 recombinant protein by ELISA and WB. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SARS-CoV-2 (COVID-19) NSP12 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SARS-CoV-2 (COVID-19) NSP12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

References for anti-SARS-CoV-2 NSP12 (RdRP) antibody (ABIN6992285)

Sun, Abriola, Niederer, Pedersen, Alfajaro, Silva Monteiro, Wilen, Ho, Gilbert, Surovtseva, Lindenbach, Guo: "Restriction of SARS-CoV-2 replication by targeting programmed -1 ribosomal frameshifting." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, Issue 26, (2021) (PubMed).

Target Details for SARS-CoV-2 NSP12 (RdRP)

Target: SARS-CoV-2 NSP12 (RdRP) (SARS-CoV-2 RdRP) (SARS-CoV-2 RNA-dependent RNA Polymerase (NSP12) (SARS-CoV-2 RdRP))

Alternative Name: SARS-CoV-2 NSP12 (SARS-CoV-2 RdRP Products)

Synonyms: pp1ab antibody, ORF1a polyprotein;ORF1ab polyprotein antibody, ORF1ab antibody

Target Type: Viral Protein

Background: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP12 interacts with nsp7 and nsp8.

Gene ID: 43740578

UniProt: P0DTC1