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Amphiregulin antibody (AA 20-100)

This Mouse Monoclonal antibody specifically detects Amphiregulin in WB, IHC, ICC and IF. It exhibits reactivity toward Human.
Catalog No. ABIN7428263

Quick Overview for Amphiregulin antibody (AA 20-100) (ABIN7428263)

Target

See all Amphiregulin (AREG) Antibodies
Amphiregulin (AREG)

Reactivity

  • 49
  • 13
  • 6
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 40
  • 23
  • 1
  • 1
  • 1
  • 1
Mouse

Clonality

  • 43
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Monoclonal

Conjugate

  • 52
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Amphiregulin antibody is un-conjugated

Application

  • 51
  • 39
  • 27
  • 26
  • 14
  • 5
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  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone

A3
  • Binding Specificity

    • 10
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
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    AA 20-100

    Purpose

    Monoclonal Antibody to Amphiregulin (AREG)

    Specificity

    The antibody is a mouse monoclonal antibody raised against AREG. It has been selected for its ability to recognize AREG in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Rat

    Purification

    Protein A + Protein G affinity chromatography

    Immunogen

    Recombinant Amphiregulin (AREG) corresdonding to Ser20~Asp100 with N-terminal His and GST Tag

    Isotype

    IgG2b kappa
  • Application Notes

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    Amphiregulin (AREG)

    Alternative Name

    AREG

    Background

    AR, CRDGF, SDGF, Colorectum Cell-Derived Growth Factor, Schwannoma-Derived Growth Factor

    UniProt

    P15514

    Pathways

    RTK Signaling, EGFR Signaling Pathway
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