IL-10 antibody (AA 1-178)

Catalog No. ABIN7425641

The Mouse Monoclonal anti-IL-10 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect IL-10 in samples from Human.

Quick Overview for IL-10 antibody (AA 1-178) (ABIN7425641)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C8

Product Details anti-IL-10 Antibody

Binding Specificity: AA 1-178

Purpose: Monoclonal Antibody to Interleukin 10 (IL10)

Specificity: The antibody is a mouse monoclonal antibody raised against IL10. It has been selected for its ability to recognize IL10 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Interleukin 10 (IL10) corresdonding to Met1~Asn178 with N-terminal His Tag

Isotype: IgG2a kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10

Background: CSIF, IL10A, TGIF, Cytokine Synthesis Inhibitory Factor

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints