IL12B antibody (AA 30-320)

Catalog No. ABIN7428812

This Mouse Monoclonal antibody specifically detects IL12B in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for IL12B antibody (AA 30-320) (ABIN7428812)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C12

Product Details anti-IL12B Antibody

Binding Specificity: AA 30-320

Purpose: Monoclonal Antibody to Interleukin 12B (IL12B)

Specificity: The antibody is a mouse monoclonal antibody raised against IL12B. It has been selected for its ability to recognize IL12B in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Interleukin 12B (IL12B) corresdonding to Val30~Ser320 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL12B

Background: P40, IL12-B, CLMF2, NKSF2, Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, Interleukin 23, P40 Subunit

UniProt: P29460

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation