IL1A antibody (AA 95-269)

Catalog No. ABIN7427081

This Mouse Monoclonal antibody specifically detects IL1A in WB, IHC, IP and ICC. It exhibits reactivity toward Horse.

Quick Overview for IL1A antibody (AA 95-269) (ABIN7427081)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C13

Product Details anti-IL1A Antibody

Binding Specificity: AA 95-269

Purpose: Monoclonal Antibody to Interleukin 1 Alpha (IL1a)

Specificity: The antibody is a mouse monoclonal antibody raised against IL1a. It has been selected for its ability to recognize IL1a in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Interleukin 1 Alpha (IL1a) corresdonding to Asp95~Gln269 (Accession # Q28385)

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.5-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-30 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL,Immunohistochemistry in paraffin section: 5-30 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL1a

Background: IL1-A, IL-1α, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha, Interleukin-1 Family Member 1

UniProt: Q28385

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints