IL-2 antibody (AA 21-155)

Catalog No. ABIN7428110

The Mouse Monoclonal anti-IL-2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect IL-2 in samples from Rat.

Quick Overview for IL-2 antibody (AA 21-155) (ABIN7428110)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C3

Product Details anti-IL-2 Antibody

Binding Specificity: AA 21-155

Purpose: Monoclonal Antibody to Interleukin 2 (IL2)

Specificity: The antibody is a mouse monoclonal antibody raised against IL2. It has been selected for its ability to recognize IL2 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Eukaryotic Interleukin 2 (IL2) corresdonding to Ala21~Gln155 with N-terminal His Tag

Isotype: IgG2a kappa

Application Details

Application Notes: Western blotting: 0.5-5 μg/mLImmunocytochemistry in formalin fixed cells: 5-30 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-30 μg/mLImmunohistochemistry in paraffin section: 5-30 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: Interleukin 2

Background: TCGF, Lymphokine, T-Cell Growth Factor, Aldesleukin

UniProt: P17108

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation