C5A antibody (AA 679-755)

Catalog No. ABIN7427909

The Mouse Monoclonal anti-C5A antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect C5A in samples from Mouse.

Quick Overview for C5A antibody (AA 679-755) (ABIN7427909)

Target: C5A (C5a) (Complement Component 5a (C5a))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C1

Product Details anti-C5A Antibody

Binding Specificity: AA 679-755

Purpose: Monoclonal Antibody to Complement Component 5a (C5a)

Specificity: The antibody is a mouse monoclonal antibody raised against C5a. It has been selected for its ability to recognize C5a in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Complement Component 5a (C5a) corresdonding to Asn679~Arg755 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for C5A

Target: C5A (C5a) (Complement Component 5a (C5a))

Alternative Name: Complement Component 5a

UniProt: P06684

Pathways: Complement System, Carbohydrate Homeostasis