Cytokeratin 5 antibody (AA 168-481)

Catalog No. ABIN7425890

The Mouse Monoclonal anti-Cytokeratin 5 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect Cytokeratin 5 in samples from Human.

Quick Overview for Cytokeratin 5 antibody (AA 168-481) (ABIN7425890)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: C5

Product Details anti-Cytokeratin 5 Antibody

Binding Specificity: AA 168-481

Purpose: Monoclonal Antibody to Cytokeratin 5 (CK5)

Specificity: The antibody is a mouse monoclonal antibody raised against CK5. It has been selected for its ability to recognize CK5 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Cytokeratin 5 (CK5) corresdonding to Glu168~Leu481 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000Immunohistochemistry: 5-20 μg/mL,1:50-200Immunocytochemistry: 5-20 μg/mL,1:50-200Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Cytokeratin 5

Background: KRT5, DDD, EBS2, K5, KRT5A, KRT5-A, Keratin 5, 58 kDa cytokeratin, Type-II keratin Kb5, Epidermolysis Bullosa Simplex Dowling-Meara/Kobner/Weber-Cockayne Types

UniProt: P13647