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HSP27 antibody (AA 2-205)

HSPB1 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal D2 unconjugated
Catalog No. ABIN7425995
  • Target See all HSP27 (HSPB1) Antibodies
    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
    Binding Specificity
    • 42
    • 34
    • 31
    • 16
    • 16
    • 12
    • 8
    • 8
    • 8
    • 6
    • 6
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-205
    Reactivity
    • 320
    • 104
    • 91
    • 19
    • 13
    • 13
    • 13
    • 10
    • 10
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 251
    • 82
    Mouse
    Clonality
    • 215
    • 117
    Monoclonal
    Conjugate
    • 168
    • 20
    • 14
    • 11
    • 10
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HSP27 antibody is un-conjugated
    Application
    • 271
    • 133
    • 110
    • 91
    • 71
    • 70
    • 69
    • 41
    • 40
    • 40
    • 15
    • 12
    • 10
    • 7
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Monoclonal Antibody to Heat Shock Protein 27 (Hsp27)
    Specificity
    The antibody is a mouse monoclonal antibody raised against Hsp27. It has been selected for its ability to recognize Hsp27 in immunohistochemical staining and western blotting.
    Purification
    Protein A + Protein G affinity chromatography
    Immunogen
    Recombinant Heat Shock Protein 27 (Hsp27) corresdonding to Thr2~Lys205 with N-terminal His Tag
    Clone
    D2
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product HSPB1 Primary Antibody
  • Application Notes
    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.
    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
    Alternative Name
    Hsp27 (HSPB1 Products)
    Background
    HSPB1, HSP-B1, CMT2F, HSP28, Hsp25, Heat Shock 27 kDa Protein 1, 28 kDa heat shock protein, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein,
    Pathways
    MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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