HIF1A antibody (AA 218-506)

Catalog No. ABIN7426046

Product Details anti-HIF1A Antibody

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Binding Specificity: AA 218-506

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a)

Specificity: The antibody is a mouse monoclonal antibody raised against HIF1a. It has been selected for its ability to recognize HIF1a in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) corresdonding to Thr218~Thr506 with N-terminal His Tag

Clone: D9

Isotype: IgG2b kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: Hypoxia Inducible Factor 1 Alpha (HIF1A Products)

Background: HIF1-A, MOP1, PASD8, Basic Helix-Loop-Helix Transcription Factor, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect