PAPPA antibody (AA 1270-1558)

Catalog No. ABIN7426052

The Mouse Monoclonal anti-PAPPA antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect PAPPA in samples from Human.

Quick Overview for PAPPA antibody (AA 1270-1558) (ABIN7426052)

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAPPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: H4

Product Details anti-PAPPA Antibody

Binding Specificity: AA 1270-1558

Purpose: Monoclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA)

Specificity: The antibody is a mouse monoclonal antibody raised against PAPPA. It has been selected for its ability to recognize PAPPA in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Pregnancy Associated Plasma Protein A (PAPPA) corresdonding to Thr1270~Cys1558 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Alternative Name: PAPPA

Background: PAPP-A, PAPA, ASBABP2, DIPLA1, IGFBP-4ase, PAPPA1, Pappalysin 1, Insulin-Like Growth Factor Binding Protein-4 Protease, Differentially Placenta 1 Expressed Protein

UniProt: Q13219