NG2 antibody (AA 559-776)

Catalog No. ABIN7426196

The Mouse Monoclonal anti-NG2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect NG2 in samples from Human.

Quick Overview for NG2 antibody (AA 559-776) (ABIN7426196)

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 6#

Product Details anti-NG2 Antibody

Binding Specificity: AA 559-776

Purpose: Monoclonal Antibody to Chondroitin Sulfate Proteoglycan 4 (CSPG4)

Specificity: The antibody is a mouse monoclonal antibody raised against CSPG4. It has been selected for its ability to recognize CSPG4 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Chondroitin Sulfate Proteoglycan 4 (CSPG4) corresdonding to His559~Pro776 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: CSPG4

Background: CSPG4, MSK16, HMW-MAA, MCSP, MCSPG, MEL-CSPG, NG2, Chondroitin Sulfate Proteoglycan 4, Melanoma chondroitin sulfate proteoglycan

UniProt: Q6UVK1

Pathways: Glycosaminoglycan Metabolic Process