CTLA4 antibody (AA 52-211)

Catalog No. ABIN7426229

The Mouse Monoclonal anti-CTLA4 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect CTLA4 in samples from Human.

Quick Overview for CTLA4 antibody (AA 52-211) (ABIN7426229)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: D4

Product Details anti-CTLA4 Antibody

Binding Specificity: AA 52-211

Purpose: Monoclonal Antibody to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4)

Specificity: The antibody is a mouse monoclonal antibody raised against CTLA4. It has been selected for its ability to recognize CTLA4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) corresdonding to Gly52~Cys211 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA4

Background: CD152, CELIAC3, CTLA4, GSE, IDDM12, Celiac Disease 3, Cytotoxic T-lymphocyte protein 4

UniProt: P16410

Pathways: Cancer Immune Checkpoints