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Cytokeratin 19 antibody (AA 245-391)

The Mouse Monoclonal anti-Cytokeratin 19 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect Cytokeratin 19 in samples from Human.
Catalog No. ABIN7427978

Quick Overview for Cytokeratin 19 antibody (AA 245-391) (ABIN7427978)

Target

See all Cytokeratin 19 (KRT19) Antibodies
Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Cytokeratin 19 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone

D1
  • Binding Specificity

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    AA 245-391

    Purpose

    Monoclonal Antibody to Cytokeratin 19 (CK19)

    Specificity

    The antibody is a mouse monoclonal antibody raised against CK19. It has been selected for its ability to recognize CK19 in immunohistochemical staining and western blotting.

    Purification

    Protein A + Protein G affinity chromatography

    Immunogen

    Recombinant Cytokeratin 19 (CK19) corresdonding to Leu245~Tyr391 with N-terminal His Tag

    Isotype

    IgG1 kappa
  • Application Notes

    Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

    Alternative Name

    CK19

    Background

    K19, KRT19, K1CS, Keratin 19, 40- kDa Keratin Intermediate Filament, Keratin, type I cytoskeletal 19

    UniProt

    P08727
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