S100A8 antibody (AA 1-93)

Catalog No. ABIN7428221

The Mouse Monoclonal anti-S100A8 antibody has been validated for WB, ICC and IF. It is suitable to detect S100A8 in samples from Human.

Quick Overview for S100A8 antibody (AA 1-93) (ABIN7428221)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: C2

Product Details anti-S100A8 Antibody

Binding Specificity: AA 1-93

Purpose: Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8)

Specificity: The antibody is a mouse monoclonal antibody raised against S100A8. It has been selected for its ability to recognize S100A8 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant S100 Calcium Binding Protein A8 (S100A8) corresdonding to Met1~Glu93 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8

Background: MRP8, MIF, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, NIF, P8, Calgranulin A, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins