ErbB2/Her2 antibody (AA 376-578)

Catalog No. ABIN7426373

This anti-ErbB2/Her2 antibody is a Mouse Monoclonal antibody detecting ErbB2/Her2 in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for ErbB2/Her2 antibody (AA 376-578) (ABIN7426373)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C16

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: AA 376-578

Purpose: Monoclonal Antibody to Receptor Tyrosine Protein Kinase erbB-2 (ErbB2)

Specificity: The antibody is a mouse monoclonal antibody raised against ErbB2. It has been selected for its ability to recognize ErbB2 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) corresdonding to Phe376~Gly578 with N-terminal His and GST Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ErbB2

Background: CD340, EGFR2, HER-2/neu, HER2, NEU, MLN 19, NGL, TKR1, p185, Epidermal Growth Factor Receptor 2, V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development