Ki-67 antibody (AA 3088-3235)

Catalog No. ABIN7426454

The Mouse Monoclonal anti-Ki-67 antibody has been validated for IHC, WB, ICC and IP. It is suitable to detect Ki-67 in samples from Human.

Quick Overview for Ki-67 antibody (AA 3088-3235) (ABIN7426454)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 6-3#

Product Details anti-Ki-67 Antibody

Binding Specificity: AA 3088-3235

Purpose: Monoclonal Antibody to Ki-67 Protein (Ki-67)

Specificity: The antibody is a mouse monoclonal antibody raised against Ki-67. It has been selected for its ability to recognize Ki-67 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Ki-67 Protein (Ki-67) corresdonding to Gly3088~Lys3235 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki-67

Background: MKI67, KIA, Ki67P, Antigen Identified By Monoclonal Antibody Ki-67

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process